Hotspot

rpoC1

LSC · 12285-14795 · 2511 bp

Back to Tetraselmis

Pi 0.46

MarkerSeek score 71.70

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2511
variable_sites: 694
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	ATTCAAGGCCGACAACAAGT	ACCAGCTCTTGTTGCTTGTT	45.0	45.0	58.0	58.2	2939 bp	0.5	58.0
rpoC1_p2	CAGATGACATTCAAGGCCGA	ACCAGCTCTTGTTGCTTGTT	50.0	45.0	58.0	58.2	2947 bp	0.5	57.9
rpoC1_p3	TCAGATGACATTCAAGGCCG	ACCAGCTCTTGTTGCTTGTT	50.0	45.0	58.0	58.2	2948 bp	0.5	57.9
rpoC1_p4	AGATGACATTCAAGGCCGAC	ACCAGCTCTTGTTGCTTGTT	50.0	45.0	58.0	58.2	2946 bp	0.5	57.9
rpoC1_p5	ATGACATTCAAGGCCGACAA	ACCAGCTCTTGTTGCTTGTT	45.0	45.0	57.8	58.2	2944 bp	0.5	56.6