Hotspot

rpoB

LSC · 8504-12259 · 3756 bp

Back to Tetraselmis

Pi 0.46

MarkerSeek score 70.50

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3756
variable_sites: 926
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	AAGGGCCAAATGCAGGTTTA	ACTCGACCTTCTAAGCCAGT	45.0	50.0	58.0	58.1	436 bp	0.5	59.2
rpoB_p2	ACACGTGAAACTGCTGGAAT	ACTCGACCTTCTAAGCCAGT	45.0	50.0	58.0	58.1	515 bp	0.5	59.1
rpoB_p3	AAGGGCCAAATGCAGGTTTA	AAGGTGTTGCCCCAAAGATT	45.0	45.0	58.0	57.9	591 bp	0.5	59.0
rpoB_p4	ACACGTGAAACTGCTGGAAT	AAGGTGTTGCCCCAAAGATT	45.0	45.0	58.0	57.9	670 bp	0.5	58.9
rpoB_p5	CGGGAGATTACCAGTTGACG	ACTCGACCTTCTAAGCCAGT	55.0	50.0	58.1	58.1	822 bp	0.5	58.8