Hotspot

rpl22

LSC · 39435-39992 · 558 bp

Back to Tetraselmis

Pi 0.447

MarkerSeek score 82.60

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 558
variable_sites: 235
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GCGCTCAATCATGCCTTTTT	TCAGTCCACTGCTCTACCAA	45.0	50.0	58.0	58.0	767 bp	0.5	59.9
rpl22_p2	GCGCTCAATCATGCCTTTTT	TCCACTGCTCTACCAACTGA	45.0	50.0	58.0	58.0	763 bp	0.5	59.9
rpl22_p3	GCGCTCAATCATGCCTTTTT	TGAGCTATCCCGGCTATCAA	45.0	50.0	58.0	58.0	746 bp	0.5	59.9
rpl22_p4	GCGCTCAATCATGCCTTTTT	TTCAGTCCACTGCTCTACCA	45.0	50.0	58.0	58.0	768 bp	0.5	59.9
rpl22_p5	GCGCTCAATCATGCCTTTTT	AACTGAGCTATCCCGGCTAT	45.0	50.0	58.0	58.0	749 bp	0.5	59.9