Hotspot

psaA

LSC · 129-2384 · 2256 bp

Back to Tetraselmis

Pi 0.457

MarkerSeek score 71.10

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: LSC
length_bp: 2256
variable_sites: 525
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.51
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	AATGAAACCGCTTGTGGTCT	TTGGTTTGTACGCATCCCAA	45.0	45.0	58.0	58.0	2898 bp	0.5	59.7
psaA_p2	ATGAAACCGCTTGTGGTCTT	TTGGTTTGTACGCATCCCAA	45.0	45.0	58.0	58.0	2897 bp	0.5	59.7
psaA_p3	AATGAAACCGCTTGTGGTCT	AAAGTTTCCTTGCCACGCTA	45.0	45.0	58.0	58.0	2721 bp	0.5	59.7
psaA_p4	ATGAAACCGCTTGTGGTCTT	AAAGTTTCCTTGCCACGCTA	45.0	45.0	58.0	58.0	2720 bp	0.5	59.7
psaA_p5	ATGAAACCGCTTGTGGTCTT	TAACAGGACCAGATGCTCCA	45.0	50.0	58.0	58.0	2838 bp	0.5	59.5