Hotspot

clpP

LSC · 7353-7976 · 624 bp

Back to Tetraselmis

Pi 0.474

MarkerSeek score 74.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 624
variable_sites: 184
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGCCGCTACACCTTCATAAC	GGTATGGCCAAGTGGTAAGG	50.0	55.0	58.0	57.9	1408 bp	0.5	59.4
clpP_p2	CCTGTTCTTGGGTCACGAAA	GGTATGGCCAAGTGGTAAGG	50.0	55.0	58.0	57.9	1500 bp	0.5	59.3
clpP_p3	TGCCGCTACACCTTCATAAC	GGTAAGGCAGAGGATTGCAA	50.0	50.0	58.0	57.9	1395 bp	0.5	58.8
clpP_p4	CCTGTTCTTGGGTCACGAAA	GGTAAGGCAGAGGATTGCAA	50.0	50.0	58.0	57.9	1487 bp	0.5	58.7
clpP_p5	CACGATACCACGGTAATCCC	GGTATGGCCAAGTGGTAAGG	55.0	55.0	57.9	57.9	1136 bp	0.5	58.4