Hotspot

chlI

LSC · 41554-42618 · 1065 bp

Back to Tetraselmis

Pi 0.412

MarkerSeek score 80.20

Primer available yes

Label chlI

Feature Metadata

label_name: chlI
feature_type: gene
region: LSC
length_bp: 1065
variable_sites: 433
pi: 0.412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI_p1	ATTGGGTCCGATGGGATTTG	GCAATAGGTCATCGGTTCGA	50.0	50.0	57.9	57.8	1858 bp	0.5	57.7
chlI_p2	ATTGGGTCCGATGGGATTTG	CCATGGCAATAGCTGGGAAA	50.0	50.0	57.9	58.2	1729 bp	0.5	57.7
chlI_p3	ATTGGGTCCGATGGGATTTG	AGCAATAGGTCATCGGTTCG	50.0	50.0	57.9	57.8	1859 bp	0.5	57.6
chlI_p4	ATTGGGTCCGATGGGATTTG	GTTGGTAGAGTGGTTGCCTT	50.0	50.0	57.9	57.7	1882 bp	0.5	57.3
chlI_p5	GGGATTTGAACCCATGACCA	GCAATAGGTCATCGGTTCGA	50.0	50.0	57.8	57.8	1846 bp	0.5	56.4