Hotspot

ccsA

IRb · 68850-69728 · 879 bp

Back to Tetraselmis

Pi 0.281

MarkerSeek score 81.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: IRb
length_bp: 879
variable_sites: 239
pi: 0.281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGAATATGCGTTTCTTCGG	GAACTAGTGACAATCCGCGT	50.0	50.0	58.0	58.0	1412 bp	0.5	59.9
ccsA_p2	GCGAATATGCGTTTCTTCGG	GGTTTTCAAAACCGGTGCAA	50.0	45.0	58.0	58.0	1586 bp	0.5	59.9
ccsA_p3	GCGAATATGCGTTTCTTCGG	GATTTTCGGGATTGACGGGA	50.0	50.0	58.0	58.0	1315 bp	0.5	59.7
ccsA_p4	GCGAATATGCGTTTCTTCGG	AGATTTTCGGGATTGACGGG	50.0	50.0	58.0	58.0	1316 bp	0.5	59.7
ccsA_p5	GCGAATATGCGTTTCTTCGG	CTACGCCGGTTTTCAAAACC	50.0	50.0	58.0	58.0	1593 bp	0.5	59.6