primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	psaA	1	AATGAAACCGCTTGTGGTCT	TTGGTTTGTACGCATCCCAA	20	20	45.0	45.0	58.0	58.0	6.3	0.0	0.036083	129	2384	2898-2898	0.500000	59.7
psaA_p2	psaA	2	ATGAAACCGCTTGTGGTCTT	TTGGTTTGTACGCATCCCAA	20	20	45.0	45.0	58.0	58.0	6.3	0.0	0.036083	129	2384	2897-2897	0.500000	59.7
psaA_p3	psaA	3	AATGAAACCGCTTGTGGTCT	AAAGTTTCCTTGCCACGCTA	20	20	45.0	45.0	58.0	58.0	6.3	0.0	0.043084	129	2384	2721-2721	0.500000	59.7
psaA_p4	psaA	4	ATGAAACCGCTTGTGGTCTT	AAAGTTTCCTTGCCACGCTA	20	20	45.0	45.0	58.0	58.0	6.3	0.0	0.043084	129	2384	2720-2720	0.500000	59.7
psaA_p5	psaA	5	ATGAAACCGCTTGTGGTCTT	TAACAGGACCAGATGCTCCA	20	20	45.0	50.0	58.0	58.0	6.3	0.0	0.067534	129	2384	2838-2838	0.500000	59.5
psaB_p1	psaB	1	GGACAACCTGGGCATTCTTT	GCAGGACTTTCCATAAGCGA	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.391587	2515	4719	2930-2930	0.500000	56.9
psaB_p2	psaB	2	GGACAACCTGGGCATTCTTT	AGCAGGACTTTCCATAAGCG	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.397250	2515	4719	2931-2931	0.500000	56.8
psaB_p3	psaB	3	CGGTGTTGCCCACTACTTAT	GCAGGACTTTCCATAAGCGA	20	20	50.0	50.0	57.6	58.0	3.9	0.0	0.420765	2515	4719	2962-2962	0.500000	56.6
psaB_p4	psaB	4	CGGTGTTGCCCACTACTTAT	AGCAGGACTTTCCATAAGCG	20	20	50.0	50.0	57.6	58.0	3.9	0.0	0.426428	2515	4719	2963-2963	0.500000	56.6
psaB_p5	psaB	5	TTGTCAAGTATCAGCGTGGG	AGTTACCACTTAGGGCTGGA	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.430665	2515	4719	2913-2913	0.500000	56.6
clpP_p1	clpP	1	TGCCGCTACACCTTCATAAC	GGTATGGCCAAGTGGTAAGG	20	20	50.0	55.0	58.0	57.9	0.0	11.0	0.071324	7353	7976	1408-1408	0.500000	59.4
clpP_p2	clpP	2	CCTGTTCTTGGGTCACGAAA	GGTATGGCCAAGTGGTAAGG	20	20	50.0	55.0	58.0	57.9	0.0	11.0	0.090989	7353	7976	1500-1500	0.500000	59.3
clpP_p3	clpP	3	TGCCGCTACACCTTCATAAC	GGTAAGGCAGAGGATTGCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.147312	7353	7976	1395-1395	0.500000	58.8
clpP_p4	clpP	4	CCTGTTCTTGGGTCACGAAA	GGTAAGGCAGAGGATTGCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.166977	7353	7976	1487-1487	0.500000	58.7
clpP_p5	clpP	5	CACGATACCACGGTAATCCC	GGTATGGCCAAGTGGTAAGG	20	20	55.0	55.0	57.9	57.9	0.0	11.0	0.197264	7353	7976	1136-1136	0.500000	58.4
rpoB_p1	rpoB	1	AAGGGCCAAATGCAGGTTTA	ACTCGACCTTCTAAGCCAGT	20	20	45.0	50.0	58.0	58.1	6.2	0.0	0.096609	10444	10593	436-436	0.500000	59.2
rpoB_p2	rpoB	2	ACACGTGAAACTGCTGGAAT	ACTCGACCTTCTAAGCCAGT	20	20	45.0	50.0	58.0	58.1	25.0	0.0	0.106573	10444	10593	515-515	0.500000	59.1
rpoB_p3	rpoB	3	AAGGGCCAAATGCAGGTTTA	AAGGTGTTGCCCCAAAGATT	20	20	45.0	45.0	58.0	57.9	6.2	0.0	0.124357	10444	10593	591-591	0.500000	59.0
rpoB_p4	rpoB	4	ACACGTGAAACTGCTGGAAT	AAGGTGTTGCCCCAAAGATT	20	20	45.0	45.0	58.0	57.9	25.0	0.0	0.134321	10444	10593	670-670	0.500000	58.9
rpoB_p5	rpoB	5	CGGGAGATTACCAGTTGACG	ACTCGACCTTCTAAGCCAGT	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.151321	10444	10593	822-822	0.500000	58.8
rpoC1_p1	rpoC1	1	ATTCAAGGCCGACAACAAGT	ACCAGCTCTTGTTGCTTGTT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.250985	12285	14795	2939-2939	0.500000	58.0
rpoC1_p2	rpoC1	2	CAGATGACATTCAAGGCCGA	ACCAGCTCTTGTTGCTTGTT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.260661	12285	14795	2947-2947	0.500000	57.9
rpoC1_p3	rpoC1	3	TCAGATGACATTCAAGGCCG	ACCAGCTCTTGTTGCTTGTT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.260661	12285	14795	2948-2948	0.500000	57.9
rpoC1_p4	rpoC1	4	AGATGACATTCAAGGCCGAC	ACCAGCTCTTGTTGCTTGTT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.262079	12285	14795	2946-2946	0.500000	57.9
rpoC1_p5	rpoC1	5	ATGACATTCAAGGCCGACAA	ACCAGCTCTTGTTGCTTGTT	20	20	45.0	45.0	57.8	58.2	0.0	0.0	0.431068	12285	14795	2944-2944	0.500000	56.6
ycf4_p1	ycf4	1	GGGGTGAATGAAAACCGACA	CTAGATTGTGGTCGCGACAA	20	20	50.0	50.0	58.4	58.0	0.0	37.3	0.397682	33868	34464	1841-1841	0.500000	56.8
ycf4_p2	ycf4	2	TGGGGTGAATGAAAACCGAC	CTAGATTGTGGTCGCGACAA	20	20	50.0	50.0	58.4	58.0	0.0	37.3	0.397682	33868	34464	1842-1842	0.500000	56.8
ycf4_p3	ycf4	3	GGGGTGAATGAAAACCGACA	GTGAAGGTGGACGAACTGTT	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.439016	33868	34464	1947-1947	0.500000	56.5
ycf4_p4	ycf4	4	TGGGGTGAATGAAAACCGAC	GTGAAGGTGGACGAACTGTT	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.439016	33868	34464	1948-1948	0.500000	56.5
ycf4_p5	ycf4	5	GGGGTGAATGAAAACCGACA	TGAAGGTGGACGAACTGTTG	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.440377	33868	34464	1946-1946	0.500000	56.5
rpl22_p1	rpl22	1	GCGCTCAATCATGCCTTTTT	TCAGTCCACTGCTCTACCAA	20	20	45.0	50.0	58.0	58.0	0.0	1.5	0.010111	39435	39992	767-767	0.500000	59.9
rpl22_p2	rpl22	2	GCGCTCAATCATGCCTTTTT	TCCACTGCTCTACCAACTGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.010111	39435	39992	763-763	0.500000	59.9
rpl22_p3	rpl22	3	GCGCTCAATCATGCCTTTTT	TGAGCTATCCCGGCTATCAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.010111	39435	39992	746-746	0.500000	59.9
rpl22_p4	rpl22	4	GCGCTCAATCATGCCTTTTT	TTCAGTCCACTGCTCTACCA	20	20	45.0	50.0	58.0	58.0	0.0	1.5	0.010111	39435	39992	768-768	0.500000	59.9
rpl22_p5	rpl22	5	GCGCTCAATCATGCCTTTTT	AACTGAGCTATCCCGGCTAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.017334	39435	39992	749-749	0.500000	59.9
tilS_p1	tilS	1	TCAGTTGGTAGAGCAGTGGA	ACAAGTGCACGTACTGTTGT	20	20	50.0	45.0	58.0	58.0	0.0	12.0	0.028223	40179	41327	1679-1679	0.500000	59.8
tilS_p2	tilS	2	TGGTAGAGCAGTGGACTGAA	ACAAGTGCACGTACTGTTGT	20	20	50.0	45.0	58.0	58.0	11.1	12.0	0.028223	40179	41327	1674-1674	0.500000	59.8
tilS_p3	tilS	3	TTGATAGCCGGGATAGCTCA	ACAAGTGCACGTACTGTTGT	20	20	50.0	45.0	58.0	58.0	9.2	12.0	0.028223	40179	41327	1696-1696	0.500000	59.8
tilS_p4	tilS	4	TTGGTAGAGCAGTGGACTGA	ACAAGTGCACGTACTGTTGT	20	20	50.0	45.0	58.0	58.0	11.1	12.0	0.028223	40179	41327	1675-1675	0.500000	59.8
tilS_p5	tilS	5	AGTTGGTAGAGCAGTGGACT	ACAAGTGCACGTACTGTTGT	20	20	50.0	45.0	58.0	58.0	0.0	12.0	0.035447	40179	41327	1677-1677	0.500000	59.7
chlI_p1	chlI	1	ATTGGGTCCGATGGGATTTG	GCAATAGGTCATCGGTTCGA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.290731	41554	42618	1858-1858	0.500000	57.7
chlI_p2	chlI	2	ATTGGGTCCGATGGGATTTG	CCATGGCAATAGCTGGGAAA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.292557	41554	42618	1729-1729	0.500000	57.7
chlI_p3	chlI	3	ATTGGGTCCGATGGGATTTG	AGCAATAGGTCATCGGTTCG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.296846	41554	42618	1859-1859	0.500000	57.6
chlI_p4	chlI	4	ATTGGGTCCGATGGGATTTG	GTTGGTAGAGTGGTTGCCTT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.343722	41554	42618	1882-1882	0.500000	57.3
chlI_p5	chlI	5	GGGATTTGAACCCATGACCA	GCAATAGGTCATCGGTTCGA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.449083	41554	42618	1846-1846	0.500000	56.4
rbcL-trnH-GTG_p1	rbcL-trnH-GTG	1	TGCAATGCACGCAGTAATTG	CGTTAGGTCTTGGACTTCCG	20	20	45.0	55.0	58.0	58.0	2.7	0.0	0.027933	51008	51434	1544-1544	0.500000	59.8
rbcL-trnH-GTG_p2	rbcL-trnH-GTG	2	GCGTGTACTCAAGCTCGTAA	CGTTAGGTCTTGGACTTCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.034692	51008	51434	1153-1153	0.500000	59.7
rbcL-trnH-GTG_p3	rbcL-trnH-GTG	3	AGCAGCTTGTGAAGTTTGGA	CGTTAGGTCTTGGACTTCCG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.064968	51008	51434	1061-1061	0.500000	59.5
rbcL-trnH-GTG_p4	rbcL-trnH-GTG	4	TCGTAACCTTGCTCGTGAAG	CGTTAGGTCTTGGACTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.068156	51008	51434	1127-1127	0.500000	59.5
rbcL-trnH-GTG_p5	rbcL-trnH-GTG	5	TGTTATTCGTGCGGCTTGTA	CGTTAGGTCTTGGACTTCCG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.089556	51008	51434	1100-1100	0.500000	59.3
ycf3_p1	ycf3	1	GTACTCTTGTGGGTACTGCG	TTGAACCAACGCGTCCTAAA	20	20	55.0	45.0	58.0	58.0	0.0	14.4	0.057002	52924	53427	1078-1078	0.500000	59.5
ycf3_p2	ycf3	2	GTACTCTTGTGGGTACTGCG	TTTGAACCAACGCGTCCTAA	20	20	55.0	45.0	58.0	58.0	0.0	14.4	0.057002	52924	53427	1079-1079	0.500000	59.5
ycf3_p3	ycf3	3	GTACTCTTGTGGGTACTGCG	CTCTGCCATAGGTGCCATTT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.070801	52924	53427	1024-1024	0.500000	59.4
ycf3_p4	ycf3	4	GTACTCTTGTGGGTACTGCG	AGTACACTGCAATCGACCAC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.088116	52924	53427	1043-1043	0.500000	59.3
ycf3_p5	ycf3	5	GTACTCTTGTGGGTACTGCG	GTACACTGCAATCGACCACT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.088116	52924	53427	1042-1042	0.500000	59.3
petA_p1	petA	1	ACTGATGCGGGAAACACATT	TCGAATAAACCGCGCTTTTC	20	20	45.0	45.0	58.1	57.5	0.0	5.6	0.604264	58535	59494	2220-2220	0.500000	55.2
petA_p2	petA	2	ACTGATGCGGGAAACACATT	CGAATAAACCGCGCTTTTCT	20	20	45.0	45.0	58.1	57.5	0.0	5.6	0.610986	58535	59494	2219-2219	0.500000	55.1
petA_p3	petA	3	CGAAAGTTACTGGCACTCCA	TCGAATAAACCGCGCTTTTC	20	20	50.0	45.0	57.8	57.5	0.0	5.6	0.677782	58535	59494	1882-1882	0.500000	54.6
petA_p4	petA	4	CGAAAGTTACTGGCACTCCA	CGAATAAACCGCGCTTTTCT	20	20	50.0	45.0	57.8	57.5	0.0	5.6	0.684504	58535	59494	1881-1881	0.500000	54.5
petA_p5	petA	5	AGCGAAAGTTACTGGCACTC	TCGAATAAACCGCGCTTTTC	20	20	50.0	45.0	58.2	57.5	6.2	5.6	0.716939	58535	59494	1884-1884	0.500000	54.3
psbD_p1	psbD	1	TGTGGGTGGATTTGGTCAAA	AGCACCAGCCCAAAATACAA	20	20	45.0	45.0	57.5	58.0	0.0	0.0	0.469542	60051	61109	1923-1923	0.500000	56.2
psbD_p2	psbD	2	TGTGGGTGGATTTGGTCAAA	CCACCAAGCAAAACCTGTTG	20	20	45.0	50.0	57.5	58.1	0.0	0.0	0.522674	60051	61109	1833-1833	0.500000	55.8
psbD_p3	psbD	3	TGTGGGTGGATTTGGTCAAA	CGCCGACAGTTAAAGTACCA	20	20	45.0	50.0	57.5	57.9	0.0	0.0	0.530137	60051	61109	1798-1798	0.500000	55.8
psbD_p4	psbD	4	TGTGGGTGGATTTGGTCAAA	AAACGAACATCTCCACCACC	20	20	45.0	50.0	57.5	58.1	0.0	0.0	0.569090	60051	61109	2318-2318	0.500000	55.4
psbD_p5	psbD	5	TGTGGGTGGATTTGGTCAAA	TCCTGGACTTACTGTTGGGT	20	20	45.0	50.0	57.5	57.9	0.0	0.0	0.573579	60051	61109	2346-2346	0.500000	55.4
ycf1_p1	ycf1	1	TAAGATGACACTCGGGCAAG	GCCTGGGGTTCATATTGGAG	20	20	50.0	55.0	57.3	58.0	0.0	0.0	0.686079	66369	68744	2848-2848	0.500000	54.5
ycf1_p2	ycf1	2	ACGTGCTCTAGTCCACTGAG	GCCTGGGGTTCATATTGGAG	20	20	55.0	55.0	58.8	58.0	11.7	0.0	0.843540	66369	68744	2792-2792	0.500000	53.3
ycf1_p3	ycf1	3	TAAGATGACACTCGGGCAAG	TAGAAAAAGCGGATCGGGTC	20	20	50.0	50.0	57.3	57.7	0.0	5.4	0.977713	66369	68744	2553-2553	0.500000	52.2
ycf1_p4	ycf1	4	TTAAGATGACACTCGGGCAAG	GCCTGGGGTTCATATTGGAG	21	20	47.6	55.0	58.0	58.0	0.0	0.0	1.040493	66369	68744	2849-2849	0.500000	51.7
ycf1_p5	ycf1	5	TAAGATGACACTCGGGCAAG	AGCCTGGGGTTCATATTGGA	20	20	50.0	50.0	57.3	58.4	0.0	0.0	1.065737	66369	68744	2849-2849	0.500000	51.5
ccsA_p1	ccsA	1	GCGAATATGCGTTTCTTCGG	GAACTAGTGACAATCCGCGT	20	20	50.0	50.0	58.0	58.0	4.7	4.9	0.013019	68850	69728	1412-1412	0.500000	59.9
ccsA_p2	ccsA	2	GCGAATATGCGTTTCTTCGG	GGTTTTCAAAACCGGTGCAA	20	20	50.0	45.0	58.0	58.0	4.7	16.4	0.016728	68850	69728	1586-1586	0.500000	59.9
ccsA_p3	ccsA	3	GCGAATATGCGTTTCTTCGG	GATTTTCGGGATTGACGGGA	20	20	50.0	50.0	58.0	58.0	4.7	0.0	0.037869	68850	69728	1315-1315	0.500000	59.7
ccsA_p4	ccsA	4	GCGAATATGCGTTTCTTCGG	AGATTTTCGGGATTGACGGG	20	20	50.0	50.0	58.0	58.0	4.7	0.0	0.043575	68850	69728	1316-1316	0.500000	59.7
ccsA_p5	ccsA	5	GCGAATATGCGTTTCTTCGG	CTACGCCGGTTTTCAAAACC	20	20	50.0	50.0	58.0	58.0	4.7	20.7	0.045903	68850	69728	1593-1593	0.500000	59.6