Hotspot

rps18

LSC · 64955-65260 · 306 bp

Back to Tetraena

Pi 0.0603

MarkerSeek score 75.20

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 25
pi: 0.0603
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AAATCGACACAATACGCCGA	AAACTCCGGGAAGGTAGAGT	45.0	50.0	58.0	57.7	1285-1305 bp	1.0	89.3
rps18_p2	AATCGACACAATACGCCGAA	AAACTCCGGGAAGGTAGAGT	45.0	50.0	58.0	57.7	1284-1304 bp	1.0	89.3
rps18_p3	AAATCGACACAATACGCCGA	AGAGTAAACTCCGGGAAGGT	45.0	50.0	58.0	57.7	1290-1310 bp	1.0	89.2
rps18_p4	AATCGACACAATACGCCGAA	AGAGTAAACTCCGGGAAGGT	45.0	50.0	58.0	57.7	1289-1309 bp	1.0	89.2
rps18_p5	AATCGACACAATACGCCGAA	GTAGTTTGTGGATCACCCGT	45.0	50.0	58.0	57.5	1535-1555 bp	1.0	86.8