Hotspot

rps18-rpl20

LSC · 65261-65604 · 344 bp

Back to Tetraena

Pi 0.113

MarkerSeek score 77.90

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 55
pi: 0.113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	CGATCTTTTCGTAGGCGGTT	AATGAAGAAAAGGGCTCCGG	50.0	50.0	58.4	58.2	1306-1334 bp	0.666667	72.1
rps18-rpl20_p2	CGATCTTTTCGTAGGCGGTT	AAAGGGCTCCGGTGTATAGA	50.0	50.0	58.4	57.8	1298-1326 bp	0.666667	72.0
rps18-rpl20_p3	CGATCTTTTCGTAGGCGGTT	GTAGTTTGTGGATCACCCGT	50.0	50.0	58.4	57.5	772-796 bp	0.666667	71.6
rps18-rpl20_p4	CGATCTTTTCGTAGGCGGTT	AAGGGCTCCGGTGTATAGAG	50.0	55.0	58.4	58.3	1297-1325 bp	0.666667	71.1
rps18-rpl20_p5	CGATCTTTTCGTAGGCGGTT	GGGCTCCGGTGTATAGAGAA	50.0	55.0	58.4	58.3	1295-1323 bp	0.666667	71.0