Hotspot

rpl33-rps18

LSC · 64322-64954 · 633 bp

Back to Tetraena

Pi 0.0548
MarkerSeek score 80.70
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
633
variable_sites
51
pi
0.0548
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 AAATCGACACAATACGCCGA AAACTCCGGGAAGGTAGAGT 45.0 50.0 58.0 57.7 1285-1305 bp 1.0 89.3
rpl33-rps18_p2 AATCGACACAATACGCCGAA AAACTCCGGGAAGGTAGAGT 45.0 50.0 58.0 57.7 1284-1304 bp 1.0 89.3
rpl33-rps18_p3 AAATCGACACAATACGCCGA AGAGTAAACTCCGGGAAGGT 45.0 50.0 58.0 57.7 1290-1310 bp 1.0 89.2
rpl33-rps18_p4 AATCGACACAATACGCCGAA AGAGTAAACTCCGGGAAGGT 45.0 50.0 58.0 57.7 1289-1309 bp 1.0 89.2
rpl33-rps18_p5 TTTTCCCAGATGCGTTGACA AAACTCCGGGAAGGTAGAGT 45.0 50.0 58.0 57.7 1910-1938 bp 1.0 86.4