Hotspot

psbE-petL

LSC · 60911-62201 · 1291 bp

Back to Tetraena

Pi 0.0634

MarkerSeek score 86.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1291
variable_sites: 119
pi: 0.0634
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGCAAAAGAACGTTCTCCTGT	AGCTGCCAGTAGAAAACCAA	42.9	45.0	58.1	57.3	1321-1366 bp	1.0	76.6
psbE-petL_p2	AGCAAAAGAACGTTCTCCTGT	AAGCTGCCAGTAGAAAACCA	42.9	45.0	58.1	57.3	1322-1367 bp	1.0	76.5
psbE-petL_p3	ACGTTCTCCTGTACTTCCAGA	AAGCTGCCAGTAGAAAACCA	47.6	45.0	58.1	57.3	1313-1358 bp	1.0	76.2
psbE-petL_p4	ACGTTCTCCTGTACTTCCAGA	AGCTGCCAGTAGAAAACCAA	47.6	45.0	58.1	57.3	1312-1357 bp	1.0	76.2
psbE-petL_p5	TCAGCAAAAGAACGTTCTCCT	AGCTGCCAGTAGAAAACCAA	42.9	45.0	57.8	57.3	1323-1368 bp	1.0	75.6