Hotspot

rbcL-accD

LSC · 53963-54564 · 602 bp

Back to Tetraena

Pi 0.187

MarkerSeek score 82.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 152
pi: 0.187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TCATGGTATGCACTTCCGTG	TAGGATTTCCAGCACCAGGA	50.0	50.0	58.0	58.0	1144 bp	0.333333	52.9
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	TAGGATTTCCAGCACCAGGA	50.0	50.0	58.0	58.0	768 bp	0.333333	52.8
rbcL-accD_p3	GGCTGCATGTGCTAGAGAAT	TAGGATTTCCAGCACCAGGA	50.0	50.0	58.0	58.0	1303 bp	0.333333	52.8
rbcL-accD_p4	TCTTCACATTCACCGTGCAA	TAGGATTTCCAGCACCAGGA	45.0	50.0	58.0	58.0	1192 bp	0.333333	52.7
rbcL-accD_p5	TTCTTCACATTCACCGTGCA	TAGGATTTCCAGCACCAGGA	45.0	50.0	58.0	58.0	1193 bp	0.333333	52.7