Hotspot

ycf3-trnS-GGA

LSC · 44379-44955 · 577 bp

Back to Tetraena

Pi 0.0558

MarkerSeek score 79.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 47
pi: 0.0558
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	GAGGCTGCATTAATCGGGAA	CTTGATTCATCCCCGCATGA	50.0	50.0	58.0	58.0	1435-1460 bp	1.0	84.7
ycf3-trnS-GGA_p2	GAGGCTGCATTAATCGGGAA	TCTTGATTCATCCCCGCATG	50.0	50.0	58.0	58.0	1436-1461 bp	1.0	84.7
ycf3-trnS-GGA_p3	GAGGCTGCATTAATCGGGAA	TGATTCATCCCCGCATGAAG	50.0	50.0	58.0	58.0	1433-1458 bp	1.0	84.7
ycf3-trnS-GGA_p4	TTGTTTCCAACGCCTCTTCA	TGATTCATCCCCGCATGAAG	45.0	50.0	58.0	58.0	1742-1769 bp	1.0	84.1
ycf3-trnS-GGA_p5	TTGTTTCCAACGCCTCTTCA	CTTGATTCATCCCCGCATGA	45.0	50.0	58.0	58.0	1744-1771 bp	1.0	84.0