Hotspot

petN-psbM

LSC · 27824-28511 · 688 bp

Back to Tetraena

Pi 0.0381

MarkerSeek score 79.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 39
pi: 0.0381
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AAGTCTTTCTTGGGCTGCTT	TGGAAGTAAACATTCTCGCA	45.0	40.0	57.6	55.0	860-871 bp	1.0	58.3
petN-psbM_p2	AGTCTTTCTTGGGCTGCTTT	TGGAAGTAAACATTCTCGCA	45.0	40.0	57.6	55.0	859-870 bp	1.0	58.3
petN-psbM_p3	AAGTCTTTCTTGGGCTGCTT	TGTTCTAGTTCCAACTGCTTTCT	45.0	39.1	57.6	58.0	820-831 bp	1.0	58.2
petN-psbM_p4	AGTCTTTCTTGGGCTGCTTT	TGTTCTAGTTCCAACTGCTTTCT	45.0	39.1	57.6	58.0	819-830 bp	1.0	58.2
petN-psbM_p5	TCTTGGGCTGCTTTAATGGT	TGGAAGTAAACATTCTCGCA	45.0	40.0	57.4	55.0	853-864 bp	1.0	56.4