Hotspot

ycf1

SSC · 127303-132771 · 5469 bp

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Pi 0.00445

MarkerSeek score 48.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5469
variable_sites: 36
pi: 0.00445
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCATACTGAAACGACTCCCA	ACTAACGTAAAAAGCGGGGG	50.0	50.0	56.9	58.2	172 bp	1.0	72.4
ycf1_p2	CCATACTGAAACGACTCCCA	CTAACGTAAAAAGCGGGGGT	50.0	50.0	56.9	58.2	171 bp	1.0	72.4
ycf1_p3	ACCATACTGAAACGACTCCC	ACTAACGTAAAAAGCGGGGG	50.0	50.0	56.9	58.2	173 bp	1.0	72.4
ycf1_p4	ACCATACTGAAACGACTCCC	CTAACGTAAAAAGCGGGGGT	50.0	50.0	56.9	58.2	172 bp	1.0	72.4
ycf1_p5	CCATACTGAAACGACTCCCA	TAACGTAAAAAGCGGGGGTC	50.0	50.0	56.9	58.2	170 bp	1.0	72.4