Hotspot

rpl22

LSC · 86122-86610 · 489 bp

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Pi 0.00692

MarkerSeek score 62.40

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 489
variable_sites: 5
pi: 0.00692
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAAGTCGGAAACCAAGTGGA	TCAATTTCCGTGGACATGCA	50.0	45.0	58.0	58.1	595-607 bp	1.0	80.6
rpl22_p2	TCCTCTTGTAGACCTTCGCA	TCAATTTCCGTGGACATGCA	50.0	45.0	58.1	58.1	665-677 bp	1.0	80.1
rpl22_p3	CAAGTCGGAAACCAAGTGGA	GGAGAATTTGCGCCCACTAT	50.0	50.0	58.0	58.3	614-626 bp	1.0	78.8
rpl22_p4	CAAGTCGGAAACCAAGTGGA	CGCCCACTATCAATTTCCGT	50.0	50.0	58.0	58.3	604-616 bp	1.0	78.7
rpl22_p5	TCCTCTTGTAGACCTTCGCA	GGAGAATTTGCGCCCACTAT	50.0	50.0	58.1	58.3	684-696 bp	1.0	78.3