Hotspot

trnL-CAA-ycf15

IRa · 147726-148464 · 739 bp

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Pi 0.00451

MarkerSeek score 61.60

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 739
variable_sites: 5
pi: 0.00451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	GCGAACCACCAGAGCTATTA	50.0	50.0	58.0	57.4	855 bp	1.0	75.6
trnL-CAA-ycf15_p2	TGAAATGGTAGACACGCGAG	GCGAACCACCAGAGCTATTA	50.0	50.0	58.0	57.4	857 bp	1.0	75.6
trnL-CAA-ycf15_p3	GCCTTGGTGGTGAAATGGTA	GCGAACCACCAGAGCTATTA	50.0	50.0	58.1	57.4	867 bp	1.0	75.0
trnL-CAA-ycf15_p4	AAATGGTAGACACGCGAGAC	TTCTTGAACAGCGAACCACC	50.0	50.0	58.0	58.7	865 bp	1.0	74.8
trnL-CAA-ycf15_p5	TGAAATGGTAGACACGCGAG	TTCTTGAACAGCGAACCACC	50.0	50.0	58.0	58.7	867 bp	1.0	74.8