Hotspot

rpl32-trnL-UAG

SSC · 116738-117490 · 753 bp

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Pi 0.0134
MarkerSeek score 60.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
753
variable_sites
15
pi
0.0134
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGTTGAAGGCTTTTTCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 873-881 bp 1.0 80.8
rpl32-trnL-UAG_p2 GCGTTGAAGGCTTTTTCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 874-882 bp 1.0 80.8
rpl32-trnL-UAG_p3 GCGTTGAAGGCTTTTTCGTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 883-891 bp 1.0 79.9
rpl32-trnL-UAG_p4 GCGTTGAAGGCTTTTTCGTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.2 57.8 882-890 bp 1.0 79.3
rpl32-trnL-UAG_p5 GCGTTGAAGGCTTTTTCGTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.2 57.8 881-889 bp 1.0 79.3