Hotspot

ycf15-trnL-CAA

IRb · 96275-97013 · 739 bp

Back to Tetradium

Pi 0.00451

MarkerSeek score 61.60

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 739
variable_sites: 5
pi: 0.00451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	GCGAACCACCAGAGCTATTA	AAATGGTAGACACGCGAGAC	50.0	50.0	57.4	58.0	855 bp	1.0	75.6
ycf15-trnL-CAA_p2	GCGAACCACCAGAGCTATTA	TGAAATGGTAGACACGCGAG	50.0	50.0	57.4	58.0	857 bp	1.0	75.6
ycf15-trnL-CAA_p3	GCGAACCACCAGAGCTATTA	GCCTTGGTGGTGAAATGGTA	50.0	50.0	57.4	58.1	867 bp	1.0	75.0
ycf15-trnL-CAA_p4	TTCTTGAACAGCGAACCACC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.7	58.0	865 bp	1.0	74.8
ycf15-trnL-CAA_p5	TTCTTGAACAGCGAACCACC	TGAAATGGTAGACACGCGAG	50.0	50.0	58.7	58.0	867 bp	1.0	74.8