Hotspot

petA-psbJ

LSC · 65195-66265 · 1071 bp

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Pi 0.00374

MarkerSeek score 61.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1071
variable_sites: 6
pi: 0.00374
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.0	58.1	1213-1215 bp	1.0	79.6
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TCCGGATTAGGTTCGTCCTT	50.0	50.0	58.0	58.1	1214-1216 bp	1.0	79.0
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.2	58.1	1210-1212 bp	1.0	78.5
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1178-1180 bp	1.0	78.2
petA-psbJ_p5	TTGGCATCTGTTGTTTTGGC	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1181-1183 bp	1.0	78.1