Hotspot

ycf4-cemA

LSC · 62824-63297 · 474 bp

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Pi 0.0162

MarkerSeek score 56.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 11
pi: 0.0162
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	ATGAGAGAGGGATCCACCAG	45.0	55.0	58.1	57.9	601-621 bp	1.0	79.9
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	TAAGGGGAATGGATGCGTTC	45.0	50.0	58.1	57.7	544-564 bp	1.0	78.1
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	CTAAGGGGAATGGATGCGTT	45.0	50.0	58.1	57.7	545-565 bp	1.0	78.0
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	GGATCCACCAGGGCAAAAAT	45.0	50.0	58.1	58.4	592-612 bp	1.0	77.3
ycf4-cemA_p5	TGCGGAATTGGCCTATTTCT	ATGAGAGAGGGATCCACCAG	45.0	55.0	57.6	57.9	608-628 bp	1.0	77.1