Hotspot

atpA-atpF

LSC · 12452-12516 · 65 bp

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Pi 0.0205

MarkerSeek score 66.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 2
pi: 0.0205
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	TGGGGCGATGAACGAAATAA	55.0	45.0	58.1	57.6	206-213 bp	1.0	77.5
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	TTGGGGCGATGAACGAAATA	55.0	45.0	58.1	57.6	207-214 bp	1.0	77.5
atpA-atpF_p3	GTCGCCTACTTGAAGTACGG	TGGGGCGATGAACGAAATAA	55.0	45.0	58.1	57.6	204-211 bp	1.0	77.5
atpA-atpF_p4	GTCGCCTACTTGAAGTACGG	TTGGGGCGATGAACGAAATA	55.0	45.0	58.1	57.6	205-212 bp	1.0	77.5
atpA-atpF_p5	GTCGCCTACTTGAAGTACGG	TTTGGGGCGATGAACGAAAT	55.0	45.0	58.1	58.5	206-213 bp	1.0	77.1