Hotspot

trnS-GCU-trnG-UCC

LSC · 8696-9667 · 972 bp

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Pi 0.0117

MarkerSeek score 59.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 972
variable_sites: 17
pi: 0.0117
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	GCAATTAAACTGAAGGACCCC	50.0	47.6	58.0	57.1	1082-1113 bp	1.0	66.5
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	GCAATTAAACTGAAGGACCCC	50.0	47.6	58.2	57.1	1140-1171 bp	1.0	65.2
trnS-GCU-trnG-UCC_p3	ACGCTTTAATCCACTCAGCC	GCAATTAAACTGAAGGACCCC	50.0	47.6	58.3	57.1	1079-1110 bp	1.0	64.4
trnS-GCU-trnG-UCC_p4	CGCTTTAATCCACTCAGCCA	GCAATTAAACTGAAGGACCCC	50.0	47.6	58.3	57.1	1078-1109 bp	1.0	64.4
trnS-GCU-trnG-UCC_p5	TCCGACGCTTTAATCCACTC	GCAATTAAACTGAAGGACCCC	50.0	47.6	57.7	57.1	1083-1114 bp	1.0	64.2