Hotspot

trnH-GUG-psbA

LSC · 82-502 · 421 bp

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Pi 0.0243
MarkerSeek score 69.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
421
variable_sites
15
pi
0.0243
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 488-535 bp 1.0 82.5
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 502-549 bp 1.0 82.3
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 501-548 bp 1.0 82.3
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 503-550 bp 1.0 82.3
trnH-GUG-psbA_p5 CGCATGGTGGATTCACAATC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.5 57.9 517-564 bp 1.0 79.5