Hotspot

psaB-V9305_pgt022

Genome · 114392-115680 · 1289 bp

Back to Tetradesmus

Pi 0.199

MarkerSeek score 88.70

Primer available yes

Label psaB-V9305_pgt022

Feature Metadata

label_name: psaB-V9305_pgt022
feature_type: igs
region: Genome
length_bp: 1289
variable_sites: 487
pi: 0.199
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 88.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB-V9305_pgt022_p1	CGAGTTGTTGGATCTTGTGC	CCTAGGACACCTCCCTTTCA	50.0	55.0	57.4	58.0	1403-1719 bp	1.0	86.8
psaB-V9305_pgt022_p2	TCTTGTGCTAAACCTTGGCT	CCTAGGACACCTCCCTTTCA	45.0	55.0	57.3	58.0	1391-1707 bp	1.0	86.4
psaB-V9305_pgt022_p3	CGAGTTGTTGGATCTTGTGC	GGAGAAAACGGGGATTCGAA	50.0	50.0	57.4	57.9	1382-1698 bp	1.0	86.4
psaB-V9305_pgt022_p4	CGAGTTGTTGGATCTTGTGC	TCCCTTTCACGGAGAAAACG	50.0	50.0	57.4	58.1	1392-1708 bp	1.0	86.2
psaB-V9305_pgt022_p5	TCTTGTGCTAAACCTTGGCT	GGAGAAAACGGGGATTCGAA	45.0	50.0	57.3	57.9	1370-1686 bp	1.0	85.9