Hotspot

V9305_pgt009-V9305_pgr006

Genome · 71083-72522 · 1440 bp

Back to Tetradesmus

Pi 0.236

MarkerSeek score 79.20

Primer available yes

Label V9305_pgt009-V9305_pgr006

Feature Metadata

label_name: V9305_pgt009-V9305_pgr006
feature_type: igs
region: Genome
length_bp: 1440
variable_sites: 574
pi: 0.236
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
V9305_pgt009-V9305_pgr006_p1	ACATATTTTATGGCGGGCGT	TCTCGTACGACTTGCATGTG	45.0	50.0	58.0	58.0	1562-1605 bp	0.6	75.6
V9305_pgt009-V9305_pgr006_p2	ACATATTTTATGGCGGGCGT	CCCGCCAAAAGGTAAGTTCT	45.0	50.0	58.0	58.1	1636-1703 bp	0.6	75.1
V9305_pgt009-V9305_pgr006_p3	ACATATTTTATGGCGGGCGT	GAACCACGATCCTTCATCCC	45.0	55.0	58.0	58.0	1973 bp	0.2	47.4
V9305_pgt009-V9305_pgr006_p4	TGTTCTGATTCTCTTGGCCG	TCTCGTACGACTTGCATGTG	50.0	50.0	57.9	58.0	1974 bp	0.2	47.0
V9305_pgt009-V9305_pgr006_p5	TGTTCTGATTCTCTTGGCCG	GAACCACGATCCTTCATCCC	50.0	55.0	57.9	58.0	2342 bp	0.2	46.8