Hotspot

psbA-V9305_pgt009

Genome · 69351-71010 · 1660 bp

Back to Tetradesmus

Pi 0.299

MarkerSeek score 87.80

Primer available yes

Label psbA-V9305_pgt009

Feature Metadata

label_name: psbA-V9305_pgt009
feature_type: igs
region: Genome
length_bp: 1660
variable_sites: 879
pi: 0.299
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 87.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-V9305_pgt009_p1	CCAAAGGCTAGATGCTTCGT	GGAGTCACAATCCATTGCCT	50.0	50.0	58.0	57.9	1742-2624 bp	0.6	74.7
psbA-V9305_pgt009_p2	CCAAAGGCTAGATGCTTCGT	TGGAGTCACAATCCATTGCC	50.0	50.0	58.0	58.2	1743-2625 bp	0.6	74.5
psbA-V9305_pgt009_p3	CCAAAGGCTAGATGCTTCGT	TTGCCTTACCACTTGGCTAC	50.0	50.0	58.0	57.8	1728-2610 bp	0.6	74.2
psbA-V9305_pgt009_p4	CCAAAGGCTAGATGCTTCGT	TCCATTGCCTTACCACTTGG	50.0	50.0	58.0	57.8	1732-2614 bp	0.6	74.0
psbA-V9305_pgt009_p5	CCAAAGGCTAGATGCTTCGT	CATTGCCTTACCACTTGGCT	50.0	50.0	58.0	58.5	1730-2612 bp	0.6	72.2