Hotspot

psbL-V9305_pgt002

Genome · 35615-36360 · 746 bp

Back to Tetradesmus

Pi 0.149
MarkerSeek score 84.50
Primer available yes
Label psbL-V9305_pgt002

Feature Metadata

label_name
psbL-V9305_pgt002
feature_type
igs
region
Genome
length_bp
746
variable_sites
209
pi
0.149
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbL-V9305_pgt002_p1 TTCACGCATTAGCAGTTCCT GGACTTGAACCCTCACGATC 45.0 55.0 57.5 58.0 1429-1831 bp 1.0 88.1
psbL-V9305_pgt002_p2 TTCACGCATTAGCAGTTCCT AAGTTGCTTGATCACCACGA 45.0 45.0 57.5 57.7 2073-2422 bp 1.0 86.2
psbL-V9305_pgt002_p3 TTCACGCATTAGCAGTTCCT CAAGTTGCTTGATCACCACG 45.0 50.0 57.5 57.7 2074-2423 bp 1.0 85.7
psbL-V9305_pgt002_p4 TTCACGCATTAGCAGTTCCT ACCAAGTTGCTTGATCACCA 45.0 45.0 57.5 57.6 2076-2425 bp 1.0 85.3
psbL-V9305_pgt002_p5 AGCCAACAGTCAAGTCAACT GGACTTGAACCCTCACGATC 45.0 55.0 57.3 58.0 1189-1240 bp 0.4 53.3