Hotspot

rpoBb-rpoBa

Genome · 30482-31129 · 648 bp

Back to Tetradesmus

Pi 0.257

MarkerSeek score 84.60

Primer available yes

Label rpoBb-rpoBa

Feature Metadata

label_name: rpoBb-rpoBa
feature_type: igs
region: Genome
length_bp: 648
variable_sites: 305
pi: 0.257
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoBb-rpoBa_p1	ACCATTGGACGCTGAACAAT	GGAGGTGTGTCACGTGATAC	45.0	55.0	58.1	58.0	1553-1570 bp	0.4	58.5
rpoBb-rpoBa_p2	ACCATTGGACGCTGAACAAT	TTGCGTTAATGGGGGTTTCA	45.0	45.0	58.1	58.0	1204 bp	0.2	47.3
rpoBb-rpoBa_p3	ACCATTGGACGCTGAACAAT	AAACTTGCGTTAATGGGGGT	45.0	45.0	58.1	58.0	1208 bp	0.2	47.2
rpoBb-rpoBa_p4	ACCATTGGACGCTGAACAAT	AACTTGCGTTAATGGGGGTT	45.0	45.0	58.1	58.0	1207 bp	0.2	47.2
rpoBb-rpoBa_p5	ACCATTGGACGCTGAACAAT	ACTTGCGTTAATGGGGGTTT	45.0	45.0	58.1	58.0	1206 bp	0.2	47.2