Hotspot

rpoC2-rps3

Genome · 22769-23401 · 633 bp

Back to Tetradesmus

Pi 0.259
MarkerSeek score 84.20
Primer available yes
Label rpoC2-rps3

Feature Metadata

label_name
rpoC2-rps3
feature_type
igs
region
Genome
length_bp
633
variable_sites
317
pi
0.259
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-rps3_p1 CGTTGCATACGTTCTACTCCT ATTGCACGTTCAGAATGGGT 47.6 45.0 57.9 58.1 1466-1478 bp 0.4 49.6
rpoC2-rps3_p2 CGTTGCATACGTTCTACTCCT GCAGGTCGTGTTCCTTTACA 47.6 50.0 57.9 58.1 1442-1454 bp 0.4 49.4
rpoC2-rps3_p3 ACTTTTGCCCTTCTTGACTCA ATTGCACGTTCAGAATGGGT 42.9 45.0 57.7 58.1 1004-1016 bp 0.4 49.1
rpoC2-rps3_p4 CGTTGCATACGTTCTACTCCT GTGCAGGTCGTGTTCCTTTA 47.6 50.0 57.9 58.1 1444 bp 0.2 37.8
rpoC2-rps3_p5 CGTTGCATACGTTCTACTCCT TGCAGGTCGTGTTCCTTTAC 47.6 50.0 57.9 58.1 1443 bp 0.2 37.8