Hotspot

psbC-atpF

Genome · 1387-2019 · 633 bp

Back to Tetradesmus

Pi 0.174

MarkerSeek score 85.20

Primer available yes

Label psbC-atpF

Feature Metadata

label_name: psbC-atpF
feature_type: igs
region: Genome
length_bp: 633
variable_sites: 213
pi: 0.174
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-atpF_p1	GAGCTACGTGTGCACCTAAA	CAAGAAGCAAGCCAAAGAGC	50.0	50.0	57.9	57.9	1117-1320 bp	0.8	81.1
psbC-atpF_p2	GAGCTACGTGTGCACCTAAA	AGGTGTATTCCGAGCAACAC	50.0	50.0	57.9	57.9	1016-1219 bp	0.8	81.0
psbC-atpF_p3	ACAATCCAACCATCACCACC	CAAGAAGCAAGCCAAAGAGC	50.0	50.0	58.1	57.9	1625-1828 bp	0.8	80.4
psbC-atpF_p4	CAATCCAACCATCACCACCA	CAAGAAGCAAGCCAAAGAGC	50.0	50.0	58.1	57.9	1624-1827 bp	0.8	80.4
psbC-atpF_p5	GGTAAACACCCCCAAAACCT	CAAGAAGCAAGCCAAAGAGC	50.0	50.0	57.9	57.9	1345-1548 bp	0.6	72.6