Hotspot

ycf1

SSC · 123744-129104 · 5361 bp

Back to Tetracme

Pi 0.0168

MarkerSeek score 47.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5361
variable_sites: 90
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTCCGCTTCTGTATTGCT	AGGTTTTATACGATCAAACGA	45.0	33.3	57.2	53.0	228 bp	1.0	43.2
ycf1_p2	AAATCGAGTTCCGCTTCTGT	AGGTTTTATACGATCAAACGA	45.0	33.3	57.5	53.0	234 bp	1.0	43.1
ycf1_p3	AAATCGAGTTCCGCTTCTGT	AAGGTTTTATACGATCAAACGA	45.0	31.8	57.5	53.9	235 bp	1.0	43.1
ycf1_p4	AAATCGAGTTCCGCTTCTGT	AGGTTTTATACGATCAAACGAA	45.0	31.8	57.5	53.9	234 bp	1.0	43.1
ycf1_p5	AAATCGAGTTCCGCTTCTGT	AGGTTTTATACGATCAAACGAAA	45.0	30.4	57.5	54.7	234 bp	1.0	43.1