Hotspot

ndhF-rpl32

SSC · 112352-113201 · 850 bp

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Pi 0.0287

MarkerSeek score 63.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 850
variable_sites: 20
pi: 0.0287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACAGGTCGTGTGAACCAAA	CAAACCATCTGCTTTGCGAC	45.0	50.0	57.9	58.3	1842-1986 bp	1.0	82.7
ndhF-rpl32_p2	ACAGGTCGTGTGAACCAAAA	CAAACCATCTGCTTTGCGAC	45.0	50.0	57.9	58.3	1841-1985 bp	1.0	82.7
ndhF-rpl32_p3	AAAAGTCCACATACGCCGAA	CAAACCATCTGCTTTGCGAC	45.0	50.0	57.8	58.3	1458-1602 bp	1.0	82.6
ndhF-rpl32_p4	GGTCGTGTGAACCAAAAACC	CAAACCATCTGCTTTGCGAC	50.0	50.0	57.8	58.3	1838-1982 bp	1.0	82.1
ndhF-rpl32_p5	ACAGGTCGTGTGAACCAAAA	TCAAACCATCTGCTTTGCGA	45.0	45.0	57.9	58.4	1842-1986 bp	1.0	82.0