Hotspot

psaJ-rpl33

LSC · 66630-67036 · 407 bp

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Pi 0.0304

MarkerSeek score 69.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 12
pi: 0.0304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTAGCGCC	ATATCTGGAAATTCCCGCCG	50.0	50.0	57.8	57.8	620-634 bp	1.0	80.4
psaJ-rpl33_p2	GCGCCGGTACTAAGTACTTT	ATATCTGGAAATTCCCGCCG	50.0	50.0	57.7	57.8	605-619 bp	1.0	79.4
psaJ-rpl33_p3	TAGCGCCGGTACTAAGTACT	ATATCTGGAAATTCCCGCCG	50.0	50.0	57.7	57.8	607-621 bp	1.0	79.0
psaJ-rpl33_p4	ACATATCTTTCCGTAGCGCC	AATTCCCGCCGATTCTTTCT	50.0	45.0	57.8	57.6	611-625 bp	1.0	78.5
psaJ-rpl33_p5	ACATATCTTTCCGTAGCGCC	ATTCCCGCCGATTCTTTCTT	50.0	45.0	57.8	57.6	610-624 bp	1.0	78.5