Hotspot

psaI-ycf4

LSC · 58955-59385 · 431 bp

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Pi 0.0194

MarkerSeek score 70.30

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 8
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCACTTGCAATGGCTTCTTT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.8	58.0	531-543 bp	1.0	82.3
psaI-ycf4_p2	AGTAGGCCTAGTCTTTCCGG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.2	58.0	550-562 bp	1.0	81.6
psaI-ycf4_p3	CCTAGTCTTTCCGGCACTTG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.3	58.0	544-556 bp	1.0	81.3
psaI-ycf4_p4	GCACTTGCAATGGCTTCTTT	ACTTGTTTTTCGAGACCCCG	45.0	50.0	57.8	58.4	533-545 bp	1.0	79.3
psaI-ycf4_p5	GCACTTGCAATGGCTTCTTT	CTTGTTTTTCGAGACCCCGT	45.0	50.0	57.8	58.4	532-544 bp	1.0	79.3