Hotspot

ndhK-ndhC

LSC · 49563-49623 · 61 bp

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Pi 0.0656

MarkerSeek score 77.20

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 4
pi: 0.0656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GGCTGGAAAGTCTTGACCAA	ATGGCGAAAGGGAGCATTAG	50.0	50.0	58.0	58.0	192 bp	1.0	81.3
ndhK-ndhC_p2	GGCTGGAAAGTCTTGACCAA	GGCGAAAGGGAGCATTAGAA	50.0	50.0	58.0	58.0	190 bp	1.0	81.2
ndhK-ndhC_p3	GGCTGGAAAGTCTTGACCAA	TTTATGCATGGCGAAAGGGA	50.0	45.0	58.0	57.9	199 bp	1.0	80.4
ndhK-ndhC_p4	GGCTGGAAAGTCTTGACCAA	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.0	57.8	200 bp	1.0	79.8
ndhK-ndhC_p5	GGCTGGAAAGTCTTGACCAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	281 bp	1.0	79.4