Hotspot

trnF-UUC-ndhJ

LSC · 47729-48305 · 577 bp

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Pi 0.0208

MarkerSeek score 55.70

Primer available yes

Label trnF-UUC-ndhJ

Feature Metadata

label_name: trnF-UUC-ndhJ
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 12
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-UUC-ndhJ_p1	CCTGGTTTACGCGAACAAAC	CCTCAACAGGGTAATCGGTG	50.0	55.0	58.0	58.0	2431-2437 bp	1.0	83.1
trnF-UUC-ndhJ_p2	CCTGGTTTACGCGAACAAAC	TTGGTCAAGACTTTCCAGCC	50.0	50.0	58.0	58.0	2852-2858 bp	1.0	82.7
trnF-UUC-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCGTTGGGCTTCGATTACC	50.0	50.0	58.1	58.1	1038-1048 bp	1.0	80.3
trnF-UUC-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TTACGTTCGCAATGTGCCTA	50.0	45.0	58.1	57.9	938-948 bp	1.0	80.1
trnF-UUC-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TTGGGCTTCGATTACCAAGG	50.0	50.0	58.1	57.9	1034-1044 bp	1.0	79.7