Hotspot

trnL-UUA-trnF-UUC

LSC · 47090-47655 · 566 bp

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Pi 0.0421

MarkerSeek score 73.30

Primer available yes

Label trnL-UUA-trnF-UUC

Feature Metadata

label_name: trnL-UUA-trnF-UUC
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 22
pi: 0.0421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUA-trnF-UUC_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	655-656 bp	1.0	82.5
trnL-UUA-trnF-UUC_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	652-653 bp	1.0	79.2
trnL-UUA-trnF-UUC_p3	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	395-618 bp	1.0	79.0
trnL-UUA-trnF-UUC_p4	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	644-645 bp	1.0	78.6
trnL-UUA-trnF-UUC_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	627-628 bp	1.0	78.2