Hotspot

trnT-ACA-trnL-UUA

LSC · 45678-46501 · 824 bp

Back to Tetracme

Pi 0.0311
MarkerSeek score 69.60
Primer available yes
Label trnT-ACA-trnL-UUA

Feature Metadata

label_name
trnT-ACA-trnL-UUA
feature_type
igs
region
LSC
length_bp
824
variable_sites
25
pi
0.0311
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACA-trnL-UUA_p1 GCGATGCTCTAACCTCTGAG CTACCGATTCCGCCATATCC 55.0 55.0 57.9 57.7 863-876 bp 1.0 80.7
trnT-ACA-trnL-UUA_p2 GGAATCGAACCGATGACCAT CTACCGATTCCGCCATATCC 50.0 55.0 57.8 57.7 895-908 bp 1.0 79.7
trnT-ACA-trnL-UUA_p3 GCGATGCTCTAACCTCTGAG GGTTTCCATACCAAGGCTCA 55.0 50.0 57.9 57.5 904-917 bp 1.0 78.8
trnT-ACA-trnL-UUA_p4 GGAATCGAACCGATGACCAT GGTTTCCATACCAAGGCTCA 50.0 50.0 57.8 57.5 936-949 bp 1.0 77.8
trnT-ACA-trnL-UUA_p5 CTAACCTCTGAGCTAAGCGG CTACCGATTCCGCCATATCC 55.0 55.0 57.5 57.7 855-868 bp 1.0 77.8