Hotspot

psbI-trnS-AGC

LSC · 7517-7608 · 92 bp

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Pi 0.0769
MarkerSeek score 81.70
Primer available yes
Label psbI-trnS-AGC

Feature Metadata

label_name
psbI-trnS-AGC
feature_type
igs
region
LSC
length_bp
92
variable_sites
7
pi
0.0769
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-AGC_p1 TCCTATCTAATGATCCAGGACGT TAGGAGAGATGGCTGAGTGG 43.5 55.0 58.0 57.9 225-226 bp 1.0 58.2
psbI-trnS-AGC_p2 TCCTATCTAATGATCCAGGACGT TGAGTGGACTAAAGCGTTGG 43.5 50.0 58.0 57.8 212-213 bp 1.0 57.7
psbI-trnS-AGC_p3 TCCTATCTAATGATCCAGGACGT GCTGAGTGGACTAAAGCGTT 43.5 50.0 58.0 58.2 214-215 bp 1.0 57.4
psbI-trnS-AGC_p4 TCCTATCTAATGATCCAGGACGT CGAGTTAATCGTACCGAGGG 43.5 55.0 58.0 57.6 175-176 bp 1.0 56.5
psbI-trnS-AGC_p5 TCCTATCTAATGATCCAGGACGT ATGGCTGAGTGGACTAAAGC 43.5 50.0 58.0 57.6 217-218 bp 1.0 55.7