Hotspot

trnH-CAC-psbA

LSC · 77-391 · 315 bp

Back to Tetracme

Pi 0.0795
MarkerSeek score 75.90
Primer available yes
Label trnH-CAC-psbA

Feature Metadata

label_name
trnH-CAC-psbA
feature_type
igs
region
LSC
length_bp
315
variable_sites
21
pi
0.0795
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-CAC-psbA_p1 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 322-373 bp 1.0 87.3
trnH-CAC-psbA_p2 ACTGCCTTAATCCACTTGGC GCTGTTGAGGCTCCATCTAC 50.0 55.0 58.2 58.1 323-374 bp 1.0 87.1
trnH-CAC-psbA_p3 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 337-388 bp 1.0 87.0
trnH-CAC-psbA_p4 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 340-391 bp 1.0 86.9
trnH-CAC-psbA_p5 ACTGCCTTAATCCACTTGGC CCTCTAGACCTAGCTGCTGT 50.0 55.0 58.2 57.9 338-389 bp 1.0 86.8