Hotspot

ndhA

SSC · 106945-109346 · 2402 bp

Back to Terniopsis

Pi 0.0405

MarkerSeek score 75.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2402
variable_sites: 176
pi: 0.0405
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCAGTTAGCATTGGGAGCAT	AGTCGAAGCACCAAAAGGAG	50.0	50.0	57.9	58.1	2637-2769 bp	0.6	71.8
ndhA_p2	CCAGTTAGCATTGGGAGCAT	GAGTCGAAGCACCAAAAGGA	50.0	50.0	57.9	58.1	2638-2770 bp	0.6	71.8
ndhA_p3	CCAGTTAGCATTGGGAGCAT	GTCGAAGCACCAAAAGGAGA	50.0	50.0	57.9	58.1	2636-2768 bp	0.6	71.8
ndhA_p4	CCAGTTAGCATTGGGAGCAT	TTTATGTGCGAGTCGAAGCA	50.0	45.0	57.9	57.9	2575-2749 bp	0.4	59.1
ndhA_p5	ACGGATGCTTTGTTGACCAT	TTTATGTGCGAGTCGAAGCA	45.0	45.0	58.1	57.9	2606-2780 bp	0.4	58.8