Hotspot

ndhF-rpl32

SSC · 100927-101233 · 307 bp

Back to Terniopsis

Pi 0.043

MarkerSeek score 64.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 307
variable_sites: 24
pi: 0.043
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCCCGCACCTATCAAAATA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	822-863 bp	0.6	67.6
ndhF-rpl32_p2	GGCCCGCACCTATCAAAATA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	823-864 bp	0.6	67.6
ndhF-rpl32_p3	GGCCCGCACCTATCAAAATA	ACCATGGCGGCTTATTCAAT	50.0	45.0	58.0	57.9	804-845 bp	0.6	67.1
ndhF-rpl32_p4	GGCCCGCACCTATCAAAATA	ATTTCACCATGGCGGCTTAT	50.0	45.0	58.0	57.9	809-850 bp	0.6	67.1
ndhF-rpl32_p5	GGCCCGCACCTATCAAAATA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.1	843-884 bp	0.6	66.9