Hotspot

accD-psaI

LSC · 55987-56413 · 427 bp

Back to Terniopsis

Pi 0.0569

MarkerSeek score 81.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 44
pi: 0.0569
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGAACCGGAAGCCCATATTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	687-727 bp	1.0	89.2
accD-psaI_p2	TGAACCGGAAGCCCATATTG	GGAAACGCCAACAACGAAAA	50.0	45.0	57.9	58.1	1347-1399 bp	1.0	88.7
accD-psaI_p3	CGCGAGTTTTGCTATGTTGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	723-763 bp	1.0	88.3
accD-psaI_p4	TGAACCGGAAGCCCATATTG	ACGGGTCGGATCCTCATTAT	50.0	50.0	57.9	58.0	840-874 bp	0.6	69.7
accD-psaI_p5	CGCGAGTTTTGCTATGTTGG	ACGGGTCGGATCCTCATTAT	50.0	50.0	58.1	58.0	876-910 bp	0.6	68.9