Hotspot

psbK-trnQ-UUG

LSC · 50001-50462 · 462 bp

Back to Terniopsis

Pi 0.0631
MarkerSeek score 85.80
Primer available yes
Label psbK-trnQ-UUG

Feature Metadata

label_name
psbK-trnQ-UUG
feature_type
igs
region
LSC
length_bp
462
variable_sites
50
pi
0.0631
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-trnQ-UUG_p1 GCTTCGGGCAATTTTGTGAA GACCAAAACCCGTTGCCTTA 45.0 50.0 57.9 58.7 485-586 bp 1.0 84.5
psbK-trnQ-UUG_p2 AAGCTTCGGGCAATTTTGTG GACCAAAACCCGTTGCCTTA 45.0 50.0 57.8 58.7 487-588 bp 1.0 84.4
psbK-trnQ-UUG_p3 GCTTCGGGCAATTTTGTGAA AAAACCCGTTGCCTTACCAC 45.0 50.0 57.9 59.0 481-582 bp 1.0 82.3
psbK-trnQ-UUG_p4 GCTTCGGGCAATTTTGTGAA ACCAAAACCCGTTGCCTTAC 45.0 50.0 57.9 59.0 484-585 bp 1.0 82.2
psbK-trnQ-UUG_p5 GCTTCGGGCAATTTTGTGAA CAAAACCCGTTGCCTTACCA 45.0 50.0 57.9 59.0 482-583 bp 1.0 82.2