Hotspot

trnS-GCU-psbI

LSC · 49348-49500 · 153 bp

Back to Terniopsis

Pi 0.0693

MarkerSeek score 82.70

Primer available yes

Label trnS-GCU-psbI

Feature Metadata

label_name: trnS-GCU-psbI
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 21
pi: 0.0693
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbI_p1	TTCGGAAAGATGGCTGAGTG	TGTTTCACTCTTCATCTTCGGA	50.0	40.9	57.9	57.4	308-313 bp	1.0	64.6
trnS-GCU-psbI_p2	CCTAAAGCGTCGGATTGCTA	TGTTTCACTCTTCATCTTCGGA	50.0	40.9	57.8	57.4	287-292 bp	1.0	64.1
trnS-GCU-psbI_p3	CCCTTTTCGGAAAGATGGCT	TGTTTCACTCTTCATCTTCGGA	50.0	40.9	58.2	57.4	313-318 bp	1.0	64.0
trnS-GCU-psbI_p4	TTCGGAAAGATGGCTGAGTG	TATCTAATGATCCGGGACGT	50.0	45.0	57.9	55.2	282-287 bp	1.0	63.8
trnS-GCU-psbI_p5	CCCTTTTCGGAAAGATGGCT	TATCTAATGATCCGGGACGT	50.0	45.0	58.2	55.2	287-292 bp	1.0	63.2