Hotspot

trnG-UCC-trnS-GCU

LSC · 48600-49259 · 660 bp

Back to Terniopsis

Pi 0.0647

MarkerSeek score 86.40

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 78
pi: 0.0647
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	ACGAATCACACTTTTACCACT	TAGCAATCCGACGCTTTAGG	38.1	50.0	55.5	57.8	732-794 bp	1.0	60.8
trnG-UCC-trnS-GCU_p2	AACGAATCACACTTTTACCACT	TAGCAATCCGACGCTTTAGG	36.4	50.0	56.2	57.8	733-795 bp	1.0	58.9
trnG-UCC-trnS-GCU_p3	TACCACTAAACTATACCCGC	TAGCAATCCGACGCTTTAGG	45.0	50.0	54.0	57.8	718-780 bp	1.0	57.1
trnG-UCC-trnS-GCU_p4	ACGAATCACACTTTTACCACT	GATTCGAACCCTCGGTACAA	38.1	50.0	55.5	57.3	770-832 bp	1.0	57.0
trnG-UCC-trnS-GCU_p5	ACGAATCACACTTTTACCACT	AACAAGACCAAGCACTCAGC	38.1	50.0	55.5	58.7	802-864 bp	1.0	56.5