Hotspot

trnC-GCA-rpoB

LSC · 29213-30177 · 965 bp

Back to Terniopsis

Pi 0.0473

MarkerSeek score 77.90

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 82
pi: 0.0473
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	GTGGCTTGGAAGTGTCAAGA	CGAGCCCATATCCTTGCTTT	50.0	50.0	58.0	58.0	2116-2117 bp	0.4	55.8
trnC-GCA-rpoB_p2	GTGGCTTGGAAGTGTCAAGA	CAGATACAGCATCGCGTTCT	50.0	50.0	58.0	58.2	1818-1819 bp	0.4	54.8
trnC-GCA-rpoB_p3	GTGGCTTGGAAGTGTCAAGA	TCAGATACAGCATCGCGTTC	50.0	50.0	58.0	58.2	1819-1820 bp	0.4	54.8
trnC-GCA-rpoB_p4	AATCAAATCGGTGGCTTGGA	CGAGCCCATATCCTTGCTTT	45.0	50.0	57.8	58.0	2126-2127 bp	0.4	54.3
trnC-GCA-rpoB_p5	ATCAAATCGGTGGCTTGGAA	CGAGCCCATATCCTTGCTTT	45.0	50.0	57.8	58.0	2125-2126 bp	0.4	54.3