Hotspot

trnT-GGU-trnE-UUC

LSC · 25815-26478 · 664 bp

Back to Terniopsis

Pi 0.0545
MarkerSeek score 85.80
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
664
variable_sites
65
pi
0.0545
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GAACCGATGACTTACGCCTT CGTGAAAGGGAGATGTCCTG 50.0 55.0 58.0 58.0 759-791 bp 1.0 86.9
trnT-GGU-trnE-UUC_p2 GAACCGATGACTTACGCCTT AAGGGAGATGTCCTGAACCA 50.0 50.0 58.0 58.0 754-786 bp 1.0 86.7
trnT-GGU-trnE-UUC_p3 CCATGGCGTTACTCTACCAC CGTGAAAGGGAGATGTCCTG 55.0 55.0 58.1 58.0 738-770 bp 1.0 86.6
trnT-GGU-trnE-UUC_p4 TGGCGTTACTCTACCACTGA CGTGAAAGGGAGATGTCCTG 50.0 55.0 58.1 58.0 735-767 bp 1.0 86.4
trnT-GGU-trnE-UUC_p5 CCATGGCGTTACTCTACCAC AAGGGAGATGTCCTGAACCA 55.0 50.0 58.1 58.0 733-765 bp 1.0 86.4